Design and validation of microfluidic structures on a Lab-on-a-CD for biomedical diagnosis

Microfluidic devices are crucial in biomedical research, enabling precise manipulation of small fluid volumes for diagnostics and drug delivery. Advances in materials and fabrication techniques have sig- nificantly improved their performance. This thesis focuses on developing and optimizing a pneumatic siphon in a microfluidic system using advanced thermoplastics and precise CNC milling.

Polymethyl Methacrylate (PMMA) was chosen for its optical clarity, strength, and cost-effectiveness, ideal for microfluidic applications. Initial designs were crafted in AutoCAD and refined with 3D modeling and simulations in Fusion 360, ensuring meticulous tool selection and parameter optimization. The primary challenge was achieving accurate fluid flow and priming within the pneumatic siphon.

The main result of this research was the valuable learning curve associated with microfluidic design and manufacturing. This study underscores the importance of iterative testing and precise engineering in microfluidic device development, revealing that practical adjustments are often needed for optimal performance. The research validates PMMA and advanced CAD/CAM tools in microfluidic fabrication, emphasizing the need for continuous refinement in design processes.

In a broader context, this study contributes to a methodology for pneumatic structures design and optimization, enhancing microfluidic system reliability and efficiency for scientific and medical applica- tions. This work highlights the critical role of material selection, precise engineering, and iterative testing in advancing microfluidic technologies, providing insights applicable across various disciplines.